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Human brown adipocytes can now be derived from pluoripotent stem cells (ES or iPS cells) by activating the classic brown adiogenic pathways (Ref 1, 2). PSdif-BA® is designed for brown adipocyte derivation from human pluoripotent stem cells (ESC or iPSC) grown as a monolayer culture. The kit contains all necessary serum-free media formulated with polypeptide differentiation factors and modulators of the key adipogenic pathways in a 3-step procedure. It is optimized to use with the serum-free and feeder-free growth medium PSGro® (StemRD cat# PGro).
Ref 1: Tseng, et al., Nature 454: 1000, 2008;
Ref 2: Nishio, et al., Cell Metab, 16: 394, 2012
Features of PSdif - BA®:
- Monolayer culture format for homogenous differentiation
- Highly-pure brown adipocytes in as few as 10 days
- Reproducible across multiple cell lines
- Protocol as easy as A – B – C
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